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ÄKTA pureNC

Non-Credit Certificate

What is ÄKTA pure?

ÄKTA pure is an easily customizable liquid chromatography system designed for protein purification. The UNICORN software comes with a library containing a wide variety of column parameters and predefined method templates to increase productivity and efficiency. High pressure and air bubble sensors are incorporated in the system to allow unattended runs. A programmable email feature is available to notify operators when an alarm is triggered.

Courses/programs listed with “NC” are non-credit courses/programs.

Who should attend the training?

These training courses are designed for researchers, laboratory technicians, manufacturing technicians, QC and QA Personnel, and Chemistry and Biology students.

Objectives of the Course

  1. Select appropriate columns for your specific project needs
  2. Prepare buffers for chromatography systems
  3. Create or edit a method for ÄKTA systems using UNICORN software
  4. Prepare and run samples on the ÄKTA systems
  5. Collect, interpret, and evaluate data
  6. Evaluate system suitability
  7. Provide General Maintenance to ÄKTA systems

REGISTER ON-CAMPUS

Please visit the Registrar’s Office on the Quincy or Plymouth Campuses.

REGISTER ONLINE

New Student? Click here to fill out the non-credit application.

After submitting the non-credit application, students will receive an email with their username and password to register online through the My QC Portal. Once you login to the MyQCPortal follow the “Current Student” instructions below for online registration.

Current Student?

Follow these steps for Online Registration

  • Go to the QCPortal and login
  • Click “Course Search” at the top of the page
  • Select “Non-Credit” from the “Term” drop down menu
  • Click the “Search” button at the bottom of the page

Courses/programs listed with “NC” are non-credit courses/programs.

Certificates

Training Course Agenda

Day One

  1. Brief introduction to liquid chromatography
  2. Introduction to the ÄKTA pure systems (hardware and software)
  3. Introduction to column resins and chromatography separation methods
  4. Hands on: setting up instruments, priming lines and purging pumps
  5. Quick column packing and prep of buffers for demos
  6. Familiarization with software
  7. Running the instrument

Day Two

  1. Column packing
  2. Buffer prep (molarity, buffer degassing)
  3. Column validation (asymmetry & HETP)
  4. Instrument preparation (system purge, ethanol check for pumps)
  5. Sample preparation, choice of loop, manual injection
  6. Fraction collection system
  7. Run samples
  8. Process results
  9. System maintenance

Day Three

  1. Trainee to repeat tasks from day two with assistance if needed
  2. Trainee given a protein sample with instruction for purification
  3. Hands-on, shut down and regular maintenance

Lifelong Learning

Training Course Agenda

Day One

  1. Brief introduction to liquid chromatography
  2. Introduction to the ÄKTA pure systems (hardware and software)
  3. Introduction to column resins and chromatography separation methods
  4. Hands on: setting up instruments, priming lines and purging pumps
  5. Quick column packing and prep of buffers for demos
  6. Familiarization with software
  7. Running the instrument

Day Two

  1. Column packing
  2. Buffer prep (molarity, buffer degassing)
  3. Column validation (asymmetry & HETP)
  4. Instrument preparation (system purge, ethanol check for pumps)
  5. Sample preparation, choice of loop, manual injection
  6. Fraction collection system
  7. Run samples
  8. Process results
  9. System maintenance

Day Three

  1. Trainee to repeat tasks from day two with assistance if needed
  2. Trainee given a protein sample with instruction for purification
  3. Hands-on, shut down and regular maintenance

Quincy

ÄKTA pureNC Courses
    •  
    • Code
    • Course
    • Credits
    • NCN 128
    • ÄKTA Pure
    ÄKTA pure is an easily customizable liquid chromatography system designed for protein purification. The UNICORN software comes with a library containing a wide variety of column parameters and predefined method templates to increase productivity and efficiency. High pressure and air bubble sensors are incorporated in the system to allow unattended runs. A programmable email feature is available to notify operators when an alarm is triggered.

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